Novel conserved motifs in Rev1 C-terminus are required for mutagenic DNA damage tolerance
نویسندگان
چکیده
منابع مشابه
Vertebrate DNA damage tolerance requires the C-terminus but not BRCT or transferase domains of REV1
REV1 is central to the DNA damage response of eukaryotes through an as yet poorly understood role in translesion synthesis. REV1 is a member of the Y-type DNA polymerase family and is capable of in vitro deoxycytidyl transferase activity opposite a range of damaged bases. However, non-catalytic roles for REV1 have been suggested by the Saccharomyces cerevisiae rev1-1 mutant, which carries a poi...
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متن کاملConserved motifs II to VI of DNA helicase II from Escherichia coli are all required for biological activity.
There are seven conserved motifs (IA, IB, and II to VI) in DNA helicase II of Escherichia coli that have high homology among a large family of proteins involved in DNA metabolism. To address the functional importance of motifs II to VI, we employed site-directed mutagenesis to replace the charged amino acid residues in each motif with alanines. Cells carrying these mutant alleles exhibited high...
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ژورنال
عنوان ژورنال: DNA Repair
سال: 2008
ISSN: 1568-7864
DOI: 10.1016/j.dnarep.2008.05.009